Western blot ladder of protein sizes verification#
Western blot ladder of protein sizes plus#

Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr).

Publishing research using ab116027? Please let us know so that we can cite the reference in this datasheet.Īb116027 has been referenced in 5 publications. Review other protein ladders in the unstained and prestained protein ladder guide.

Western blot ladder of protein sizes plus#

Precision Plus Protein Unstained Recombinant Protein Standards are Strep-tagged, enabling immunodetection and molecular weight determination on western blots.

Recommended loading: ~2 - 3 µl for western blots Unstained natural protein standards allow accurate MW determination with uniform band intensities on SDS-PAGE gels stained with Coomassie Blue or zinc. Precision Plus Protein WesternC Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate.

Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.

Rainbow molecular markers are available for both chemiluminescence based detection and fluorescence detection applications. Full-, High-, and Low-Range (left to right). If the Western blot is not behaving as expected, our troubleshooting guide may help isolate the problem. Rainbow Molecular Weight Markers are available in three size ranges for use with polyacrylamide gel electrophoresis (SDS-PAGE). The robust nature of the antigen-antibody interaction allows the presence of specific proteins and peptides to be detected from complex mixtures.

Ready-to-use: Supplied in a loading buffer for direct loading on gels. Western blotting is a staple technique of the molecular biology lab.

Quality Control: Tested in polyacrylamide gel electrophoresis and Western. The ladder is supplied in gel loading buffer and is ready to use.ĭo not heat, dilute, add reducing agent before loading. Usage: For each application, 5-15l is needed, depending on the size of the wells.

Western blot ladder of protein sizes verification#

This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Improved Dual Color Standards are brighter than the competition on both gels and blots.Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. 8 blue-stained bands and 2 pink reference bands (25 and 75 kD).Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD).Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.